Myogenic progenitor cell transplantation for muscle regeneration following hindlimb ischemia and reperfusion

Abstract Background Muscle is severely affected by ischemia/reperfusion injury (IRI). Quiescent satellite cells differentiating into myogenic progenitor (MPC) possess a remarkable regenerative potential. We herein established model of local application MPC in murine hindlimb to study cell engraftment and differentiation required for muscle regeneration. Methods A clamping (C57b/6 J) ischemia was induce IRI skeletal muscle. After 2 h (h) warm ischemic time (WIT) reperfusion, reporter protein expressing (TdTomato or Luci-GFP, 1 × 10 6 cells) obtained from isolated were injected intramuscularly. Surface marker expression potential analyzed vitro flow cytometry assay. In vivo bioluminescence imaging histopathologic evaluation biopsies performed quantify fate, Results 2h WIT induced severe on muscle, fiber regeneration as per histopathology within 14 days after injury. Bioluminescence demonstrated signals animals controls over the period (75 days). detected at injection site increased time. TdTomato myofibers visible host tissue postoperative 14, respectively, suggesting that differentiated fibers. Higher found compared without ischemia, indicative enhanced growth and/or IRI-affected antagonizing damage caused IRI. Conclusion WIT-induced requests numbers engraft persist, possible rational therapy antagonize Further investigations are needed evaluate capacity therapeutic advantage setting limb